ZIA BC 011294 (ZIA) | |||
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Title | Conditional manipulation of hnRNPLL expression in murine models | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Oberdoerffer, Shalini | NCI Program Director | N/A |
Cancer Activity | N/A | Division | CCR |
Funded Amount | $227,493 | Project Dates | 10/01/2009 - N/A |
Fiscal Year | 2011 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Autoimmune Diseases (35.0%) Cancer (100.0%) Digestive Diseases (100.0%) |
N/A | ||
Research Type | |||
Normal Functioning Endogenous Factors in the Origin and Cause of Cancer |
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Abstract | |||
Mice for conditional hnRNPLL expression or deletion have been generated. HnRNPLL cDNA was knocked into the ROSA26 locus downstream of a lox P flanked STOP cassette. These mice were crossed to mice with Cre-recombinase expression from the CD19 promoter, leading to hnRNPLL expression early in B cell development. We have confirmed that hnRNPLL is expressed, with the expected effect on CD45 alternative splicing. In addition, we observe some alterations in B cell development that are being explored in greater detail. We have further generated mice for Cre-recombinase mediated hnRNPLL deletion. These mice are being bred to homozygosity and will be crossed to mice expressing Cre from either CD19, for deletion in B cells, or Lck, for deletion in T cells. |